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Update viromes.md
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asierFernandezP authored Oct 11, 2024
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## Step 1 - Initial virus discovery

First, we run **geNomad** to identify viral sequences in our data. We use the ``--enable-score-calibration`` option to compute false discovery rates, allowing us to set a threshold to achieve a desired proportion of false positives (here we will use an FDR < 0.05). Further, we add the use the ``--cleanup`` flag to remove intermediate files and the ``--disable-find-proviruses`` option to avoid geNomad performing an initial prunning to remove potential contaminant host regions from proviral sequences (we will do this in the next step with CheckV).
First, we will run **geNomad** to identify viral sequences in our dataset. We will utilize the ``--enable-score-calibration`` option to compute false discovery rates (FDR). For this practical exercise, no specific FDR thresholds will be applied. Further, we will add the ``--cleanup`` flag to remove intermediate files and the ``--disable-find-proviruses`` option to avoid geNomad performing an initial prunning to remove potential contaminant host regions from proviral sequences (we will do this in the next step with CheckV).

**Note:** The following command takes about 10 minutes. You can also continue with the results in ``/data/precomputed/virome/genomad_results/``.
```bash
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