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@@ -56,18 +56,27 @@ usage: ViralMSA.py [-h] -s SEQUENCES -r REFERENCE -e EMAIL -o OUTPUT [-a ALIGNER | |
-u, --update Update ViralMSA (default: False) | ||
``` | ||
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### GenBank Accession Number | ||
For the reference genome, you can provide a GenBank accession number, such as the following: | ||
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``` | ||
ViralMSA.py -e [email protected] -s sequences.fas -o output -r NC_045512 | ||
``` | ||
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### Preselected Reference Genomes | ||
For specific viruses of interest, you can simply use their name, and we have provided what we believe would be a good choice of reference genome, such as the following: | ||
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``` | ||
ViralMSA.py -e [email protected] -s sequences.fas -o output -r SARS-CoV-2 | ||
``` | ||
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Our preselected viral reference genomes can be found in the following GitHub repository: | ||
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https://github.com/Niema-Lab/Reference-Genomes | ||
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If you would like to contribute a new or updated viral reference genome to our collection, please feel free to submit a [GitHub Issue](https://github.com/Niema-Lab/Reference-Genomes/issues/new) or a [Pull Request](https://github.com/Niema-Lab/Reference-Genomes/pulls) with all relevant information. | ||
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### Local File | ||
If you have a local reference genome you would like to use, you can provide the path to a FASTA file with a single sequence, such as the following: | ||
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``` | ||
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