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whole genome pairwise comparison

This repository hosts codes and results for pairwise whole genome comparison between any two genome (moslty maize) assemblies.

  • minimap2 was used to align one assembly to another;
  • blat chain/net tools were used to process alignment resuls and build synteny chains/nets;
  • bcftools and GATK4 were used to call variants;
  • snpEff was used to evaluate variant effects on sytenic genes

See this page for a sumamry of comparing four maize de novo assemblies (W22, Mo17, PH207 and PHB47) to the B73 reference.

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whole genome comparison pipeline

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