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#### DArT Sample Submission | ||
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<!-- Grzegorz Uszynski and Puthick Hok - Diversity Arrays Technology DArT --> | ||
The DArT genotyping lab is heavily used world wide when it comes to plant genotyping. With over 1200 available organisms and species, client base on every continent and already many million samples processed, DArT provides services for several generic and bespoke genotyping technologies and solutions. Processes of sample tracking and fast data delivery are at the core of the ordering system developed at DArT. The ordering system is tightly integrated with DArTdb - DArT's custom LIMS operational system, which drives laboratory, quality, and analytical processes. | ||
The [Diversity Arrays Technology (DArT)](https://www.diversityarrays.com/) genotyping lab is heavily used world wide when it comes to plant genotyping. With over 1200 available organisms and species, a client base on every continent, and many millions of samples processed, DArT provides services for several generic and bespoke genotyping technologies and solutions. Processes of sample tracking and fast data delivery are at the core of the ordering system developed at DArT. The ordering system is tightly integrated with DArTdb - DArT's custom LIMS operational system, which drives laboratory, quality, and analytical processes. | ||
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Diversity Arrays Technology was a part of BrAPI community since its inception. DArT developers have worked with the BrAPI community contributing to various aspects of the API specification. One key aspect was establishing a standard API for sending sample metadata to the lab for genotyping. This solution eliminates much of the human error involved with sending samples to an external lab and also allows for an automated process of sample batch transfers. Beyond sample submission, the current implementation also allows for an order status verification, automated data discovery, and data downloads. Data are delivered as standard data packages with self-describing metadata. | ||
Diversity Arrays Technology has been a part of BrAPI community since its inception. DArT developers have worked with the BrAPI community contributing to various aspects of the API specification. One key aspect was establishing a standard API for sending sample metadata to the lab for genotyping. This solution eliminates much of the human error involved with sending samples to an external lab, and also allows for an automated process of sample batch transfers. The current implementation also allows for an order status verification, automated data discovery, and data downloads. Data are delivered as standard data packages with self-describing metadata. | ||
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The current BrAPI implementation at DArT is in production and it is compatible with the newest BrAPI specification. Further details about DArT's ordering system can be found at [DArT Ordering System](https://ordering.diversityarrays.com) and also at [DArT Help](https://help.diversityarrays.com/docs/ordering). |
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#### DArTView | ||
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<!-- Moses N --> | ||
[DArTView](https://software.kddart.com/kdxplore/dartview/dartviewdocs/KDXplore-DartView.html) is a desktop application for marker data curation via metadata filtering. DArTView enables genotype variant data visualization and users can easily identify trends or correlations within their data using the tool. Its primary goal is to overcome tedious manual calculation of marker data through common spreadsheet applications like Excel. Users are able to import marker data from csv files, but DArTView has been recently enhanced to be BrAPI compatible. Users can now use any BrAPI compatible server as an input data source. BrAPI provides a consistent data standard across databases and data resources. DArTView's compatibility with BrAPI also ensures easy integration with other tools and pipelines that would use DArTView for marker filtering and exploration. | ||
[DArTView](https://software.kddart.com/kdxplore/dartview/dartviewdocs/KDXplore-DartView.html) is a desktop application for marker data curation via metadata filtering. DArTView enables genotype variant data visualization designed such that users can easily identify trends or correlations within their data. The primary goal of the tool is to overcome tedious manual calculation of marker data through common spreadsheet applications like Excel. Users are able to import marker data from csv files, but DArTView has been recently enhanced to be BrAPI compatible. BrAPI provides a consistent data standard across databases and data resources, which allows DArTView to use any BrAPI-compatible server as an input data source. DArTView's compatibility with BrAPI also ensures easy integration with other tools and pipelines that would use DArTView for marker filtering and exploration. | ||
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Initially developed by Diversity Arrays Technology (DArT), the tool is gaining popularity within the breeding community, especially in Africa. Future releases will focus on enhancing the BrAPI compatibility, making it accessible to more breeders and researchers in the region. A web enabled version of DArTView is in development. This new version will allow for further collaboration opportunities with other interested partners who would like to integrate it as part of their pipelines. | ||
Initially developed by DArT, the tool is gaining popularity within the breeding community, especially in Africa. Future releases will focus on enhancing the BrAPI compatibility, making it accessible to more breeders and researchers in the region. A web enabled version of DArTView is in development. This new version will allow for further collaboration opportunities with other interested partners who would like to integrate it as part of their pipelines. |
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#### PHG | ||
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<!-- Lynn J. --> | ||
The [Practical Haplotype Graph](https://www.maizegenetics.net/phg) (PHG) is a graph-based computational framework that represents large-scale genetic variation and is optimized for plant breeding and genetics [@doi:10.1093/bioinformatics/btac410]. Using a pangenome approach, each PHG stores haplotypes (the sequence of part of an individual chromosome) to represent the collected genes of a species. This allows for a simplified approach for dealing with large scale variation in plant genomes. The PHG pipeline provides support for a range of genomic analyses and allows for the use of graph data to impute complete genomes from low density sequence or variant data. | ||
The [Practical Haplotype Graph (PHG)](https://www.maizegenetics.net/phg) is a graph-based computational framework that represents large-scale genetic variation and is optimized for plant breeding and genetics [@doi:10.1093/bioinformatics/btac410]. Using a pangenome approach, each PHG stores haplotypes (the sequence of part of an individual chromosome) to represent the collected genes of a species. This allows for a simplified approach for dealing with large scale variation in plant genomes. The PHG pipeline provides support for a range of genomic analyses and allows for the use of graph data to impute complete genomes from low density sequence or variant data. | ||
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Users access the crop databases either with direct calls to the PHG embedded server or indirectly using the rPHG library from an R environment. The PHG server accepts BrAPI queries to return information on sample lists and the variants used to define the graph's haplotypes. In addition, PHG users utilize the BrAPI Variant Sets endpoint query to return links to VCF files containing haplotype data. Work on the PHG is ongoing and it is expected to support additional BrAPI endpoints that allow for fine tuned slicing genotypic data in the near future. |
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