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small RNA-Seq pipeline (paired-end/single-end) ---------------------------------------------- The small RNA-seq pipeline requires the following software fastqc-0.11.8-1 multiqc-1.7-2 bbmap-38.22-1 dnapi-1.1-3 cutadapt-2.1-0 umi-tools-0.5.5 seqkit-0.10.1-1 fastx_toolkit-0.0.14-7 bowtie-1.2.2-1 mirdeep2-2.0.0.8 File "smallseq.sh" contains the code for the mirna expression profiling using miRDeep2 Example paired-end dataset: PRJNA490335 ---------------------------------------------------------------------------------------------------------------------------------- References 1. Pacholewska A et al. Differential Expression of Serum MicroRNAs Supports CD4+ T Cell Differentiation into Th2/Th17 Cells in Severe Equine Asthma. Genes (Basel) 2017 Dec; 8(12): 383. PMCID: PMC5748701 2. Friedländer M R et al. miRDeep2 accurately identifies known and hundreds of novel microRNA genes in seven animal clades. Nucleic Acids Res. 2012 Jan; 40(1): 37–52. PMCID: PMC3245920 3. Lin J et al. Network-based integration of mRNA and miRNA profiles reveals new target genes involved in pancreatic cancer. Mol Carcinog 2019 Feb;58(2):206-218. PMID: 30294829
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