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29.02.2024: pictures update
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wisstock committed Feb 29, 2024
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14 changes: 7 additions & 7 deletions README.md
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Expand Up @@ -29,7 +29,7 @@ Provides functions for preprocessing multi-channel fluorescence acquisitions:
- If the `photobleaching correction` option is selected, the image will undergo correction with exponential (method `exp`) or bi-exponential (method `bi_exp`) fitting.
- If the `crop ch` option is selected, only a selected range of channel frames will be saved (corresponding to start and stop indexes from `crop range`).

![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_0.png)
![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_00.png)

## Red-green series
Primary method for detecting fluorescent-labeled targets redistribution in time. Returns a series of differential images representing the intensity difference between the current frame and the previous one as new image with the `_red-green` suffix.
Expand All @@ -40,7 +40,7 @@ Parameters:
- `space frames` - number of frames between the last left and first right frames.
- `right frames` - number of subsequent frames for pixel-wise averaging.

![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_1.png)
![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_11.png)

## Up masking
Generates labels for insertion sites (regions with increasing intensity) based on `-red-green` images. Returns labels layer with `_up-labels` suffix.
Expand All @@ -52,12 +52,12 @@ Parameters:
- `opening footprint` - footprint size in pixels for mask filtering with morphology opening (disabled if 0).
- `save mask` - if selected, a total up mask (containing all ROIs) will be created with the `_up-mask` suffix.

![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_2.png)
![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_22.png)

## Intensity masking
Extension of __Up Masking__ widget. Detects regions with increasing (`masking mode` - `up`) or decreasing (`masking mode` - `down`) intensity in `-red-green` images. Returns a labels layer with either `_up-labels` or `_down-labels` suffix, depending on the mode.

![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_3.png)
![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_33.png)


# Traffic monitoring with pH-sensitive tag
Expand Down Expand Up @@ -96,9 +96,9 @@ Additionally, you can save ROI intensity profiles as .csv using the `save data f

_Note: The data frame will contain information for all ROIs; amplitude filtering and crop options pertain to plotting only._

Absolute intensity | ΔF/F0
Absolute intensity | ΔF/F0
:-------------------------:|:-------------------------:
![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_4.png) | ![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_5.png)
![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_44.png)|![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_55.png)


## Labels stat profiles
Expand All @@ -112,4 +112,4 @@ The `stat method` provides methods for estimation intensity and errors:
- `iqr` - interquartile range.
- `ci` - 95% confidence interval for t-distribution.

![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_6.png)
![](https://raw.githubusercontent.com/wisstock/domb-napari/master/images/pic_66.png)
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