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Merge pull request #143 from MikeLippincott/update_readme
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update readme
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MikeLippincott authored Apr 30, 2024
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[![DOI](https://zenodo.org/badge/611024034.svg)](https://zenodo.org/doi/10.5281/zenodo.11075332)

# pyroptosis_signature_data_analysis
Analysis Repository for the Interstellar project. For data processing for this project please see [pyroptosis_signature](https://github.com/WayScience/pyroptosis_signature)
Analysis Repository for the Interstellar project. For image analysis and image-based profiling for this project, please see [pyroptosis_signature_image_profiling](https://github.com/WayScience/pyroptosis_signature_image_profiling)

### Workflow:

Cells were incubated for 1h in the inhibitors, and then incubated for 6h in each inducer.
For more information on plate layout and experimental design please see [pyroptosis_signature](https://github.com/WayScience/pyroptosis_signature).
For more information on plate layout and experimental design please see [pyroptosis_signature_image_profiling](https://github.com/WayScience/pyroptosis_signature_image_profiling).


# Treatments of Interest (All in this table are with DMSO 0.025% inhibitor)
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| LPS + Nigercin | 1 * 1 = (1) | 1ug/mL+10ug/mL | Z-VAD-FMK | 1 | 100uM | Induce Pyroptosis <br> Inhibit Inflamasome |
| LPS + Nigercin | 1 * 1 = (1) | 1ug/mL+10ug/mL | Disulfiram | 1 | 1uM | Induce Pyroptosis <br> Inhibit Inflamasome |
| Disulfiram | 3 | 0.1uM <br> 1uM <br> 2.5uM | DMSO | 1 | 0.025% | Pyroptosis Inhibitor |


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