This pipeline is designed to process and analyze RNA sequencing (RNA-seq) data. The pipeline utilizes Nextflow, a high-performance workflow tool that allows for easy and reproducible data processing. The steps involved in the pipeline are as follows:
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Quality control and trimming of raw sequencing reads: This step involves checking the quality of raw sequencing data and trimming low-quality regions.
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Mapping reads: In this step, reads are aligned by KALISTO (pseudoalignment.
By using Nextflow, this pipeline can be easily run on a local workstation or a high-performance computing cluster, allowing for efficient and scalable processing of large scRNA-seq datasets. The pipeline can be easily adapted or extended to include additional steps or tools as needed.