Analysis of tRNA abundances and modifications using native RNA nanopore sequencing
This repository contains:
- conf/: MinKNOW alternative configurations enhanced capture of tRNA reads
- ref/: reference files
- src/: source code
Yes, it is possible, both for SQK-RNA002 and SQK-RNA004 kits. Please take a look here.
If you find this work useful, please cite:
Lucas MC*, Pryszcz L*, Medina R, Milenkovic I, Camacho N, Marchand V, Motorin Y, Ribas de Pouplana L and Novoa EM. Quantitative anaysis of native tRNA populations using direct RNA nanopore sequencing. Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01743-6