Pipeline

Main scripts

1.1.Genome_RAST_to_Evo.pl

Converts RAST format to EvoMining format.

startEvoMining.pl

Initialize EvoMining with custom genome, natural products and central pathways databases.
Blast outputs:
pscp.blast Conserved-Enzymes DB as queries vs Genomes as database
vuelta.blast GenomesDB as queries vs Conserved-EnzymesDB database

Processed blast outputs:
-pscplos17.blast.uniq Finds best Hit of Conserved-Enzymes from file pscp.blast

-vueltalos17.blast.bbh Only Best Bidirectional Hits

-vueltalos17.blast.uniq Finds Best Hit of Genomes as queries vs Conserved-EnzymesDB database from file
vuelta.blast

reparaHEADER.pl

Blastp to detect expanded families from central pathways on the genome DB provided.

heatplot.pl

Draw heat plot of expanded families on browser.

np_hits_from_cp.pl

Search recruitments of expanded families on natural products Database.

align_shave_tree.pl

Align trees of expanded families eriched with natural products.

color_tree.pl

Draw trees marking antismash hits, MiBig NPs, Central pathways BBH and EvoMining hits.